Table 1.

Kinetic analysis of the purified recombinant COMT

Substrates	K m [μM]	K cat [s−1]	Catalytic efficiency K cat/K m
Caffeic acid	40.5 ± 6.6	(35.3 ± 5.7) × 10−3	872.8
Resveratrol	44.9 ± 3.2	(12.8 ± 1.4) × 10−3	283.9

Determination of the K _m and K _cat of the COMT activity for caffeic acid to ferulic acid and ROMT activity for resveratrol to pinostilbene. Purified COMT (4 μg) was incubated with different concentrations of substrate for 30 min at 37 °C. The assay conditions are described in the “Methods” section. The data represent the mean S.D. of triplicate experiments