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. 2017 Feb 16;16:79. doi: 10.1186/s12936-017-1716-0

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Expression of 6-Cys domain proteins: Pfs41, Pfs38, Pfs12 and PfMSP-1₆₅. a Schematic showing the organization of the above-mentioned Plasmodium proteins. Arrows mark the regions that were expressed in Escherichia coli. b Coomassie stained SDS-PAGE and immunoblots showing the purified Pfs41, Pfs38, Pfs12 and PfMSP-1₆₅. Immunoblots were performed using mouse monoclonal anti-his antibody. c Recognition of native Pfs41, Pfs38 and Pfs12 proteins in parasite lysate from asexual blood stages using their respective antibodies generated against recombinant proteins