FIG. 3.

Tenogenesis of hASCs in plate cultures. (A) hASCs were plated on 2D tissue culture plastic, and treated with or without 10 ng/mL TGF-β3 in BM containing one of the following types of supplements at 10% v/v: FBS, 1 mg/mL tECM solution (tECM), or 1 mg/mL collagen type I solution (Col I). (B, C) Real-time PCR analysis of (B) scleraxis (SCX) and (C) tenascin-C (TNC) expression levels. tECM treatment up-regulated SCX expression in the presence of TGF-β3, and increased TNC expression with or without TGF-β3. (D) Western blot assay showed consistent difference in SCX protein. *p < 0.05; **p < 0.01; N = 3. PCR, polymerase chain reaction; TGF, transforming growth factor. Color images available online at www.liebertpub.com/tea