FIG. 5.

Dgcr8^−/− NSCs exhibit increased DNA damage. (A–A″) Representative images of γH2AX immunostaining in Dgcr8^+/− NSCs. (A) Immunostaining of γH2AX (red), (A′) DAPI staining, and (A″) merged. Scale bars, 50 μm. (B–B″) Representative images of γH2AX immunostaining in Dgcr8^−/− NSCs. (B) Immunostaining of γH2AX (red), (B′) DAPI staining, and (B″) merged. Scale bars, 50 μm. (C) Quantification of γH2AX positive cells of Dgcr8^+/− and Dgcr8^−/− NSCs. n = 3 independent biological repeats. P < 0.05, two-tailed Student's t-test. (D) Immunoblotting of Dgcr8 and γH2AX in Dgcr8^+/− and Dgcr8^−/− NSCs. Immunoblotting of GAPDH and Ponceau S staining are used as loading controls. (E, F) Representative Comet assay images of (E) Dgcr8^+/− and (F) Dgcr8^−/− NSCs. (G) A Box-and-Whisker plot quantifies comet assays of Dgcr8^+/− (n = 126) and Dgcr8^−/− (n = 121) NSCs. P < 0.001, two-tailed Student's t-test. Color images available online at www.liebertpub.com/scd