Skip to main content
. 2017 Feb 1;27(1):23–35. doi: 10.1089/nat.2016.0621

FIG. 3.

FIG. 3.

AP1153 uptake by PANC-1 cells is mediated through the CCKBR. Representative 3D confocal images showing AP1153 distributions in cultured PANC-1 cells that overexpress CCKBR (A–C), PANC-1 wild-type cells (D), and PANC-1 cells where the CCKBR has been knocked down by stable shRNA transfection (E). The aptamer distributions are shown in green, while quantified voxels are shown yellow. Blue represents cell nuclei. Uptake of a first-generation aptamer, AP38, by PANC-1 cells overexpressing CCKBR (F), wild-type (G), and CCKBR knockdown cells (H) is significantly less than AP1153 (L). All PANC-1 cells treated only with vehicle (I–K) show minimal background fluorescence. Integrated signal intensities from cell volumes (M) show that the CCKBR overexpressing cells take up significantly more AP1153 than either wild-type cells (WT) or CCKBR knockdown cells (KO) [*P < 0.05]. 3D, three dimensional.