Angiogenic testing of TSBTs: chorioallantoic membrane assay. (A) Representative images of negative control (nylon membrane), nylon loaded with VEGF 200 ng/mL and engineered matrix scaffold TSBT placed in ovo at the end of 7 days of incubation. (B) Number of vessels attracted toward the matrices at days 0 and 7 postimplantation, significant differences between all groups at day 7. *p < 0.05 (day 0: n = 4 for nylon, n = 4 for nylon + VEGF, n = 3 for TSBT scaffold; day 7: n = 3 for nylon, n = 4 for nylon + VEGF, n = 3 for TSBT scaffold). VEGF, vascular endothelial growth factor.