FIG. 8.

Microsphere incorporation in TGF-β1-treated rings. (A, F) Control (untreated) rings. (B, G) Rings without microspheres cultured with exogenous TGF-β1 (10 ng/mL). Rings with unloaded microspheres are (C, H) untreated or (D, I) treated with exogenous TGF-β1. (E, J) Rings with TGF-β1-loaded microspheres but without exogenous TGF-β1. (A–E) Hematoxylin and eosin stain and (F–J) Picrosirius Red/Fast Green stain, (F–J; red = collagen, green = counterstain). Example microspheres marked with asterisks. Scale = 100 μm. TGF-β1, transforming growth factor beta 1. Color images available online at www.liebertpub.com/tea