Figure 5.

HOXA1 is a direct target of miR-99a in nasopharyngeal carcinoma.

Notes: (A) Wild type and mutant miR-99a target sequences of the 3′-UTR of HOXA1 mRNA; (B–D) HOXA1 mRNA (B) and protein (C) expression and luciferase activities (D) were measured by real-time RT-PCR, Western blotting, and luciferase reporter assays in CNE-2 and HONE-1 cells after miR-99a mimics or inhibitor transfection. *P<0.05.

Abbreviations: RT-PCR, reverse transcriptase polymerase chain reaction; 3′-UTR, 3′-untranslated region; CMV, cytomegalovirus; WT, wild-type; MT, mutant type.