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. Author manuscript; available in PMC: 2017 Feb 16.
Published in final edited form as: Nat Methods. 2016 Jun 6;13(8):673–678. doi: 10.1038/nmeth.3894

Figure 1. GFE3 specifically ablates Gephyrin.

Figure 1

(a–c) Immunostaining showing Gephyrin (green) and PSD-95 (magenta) localization at inhibitory and excitatory synapses, respectively, after expression of GPHN.FingR (a), RandE3 (b) or GFE3 (c) in cultured rat cortical neurons. (d) Western blot showing the expression of Gephyrin, the alpha1 subunit of the GABAA receptor, PSD-95, the GluA1 subunit of the AMPA receptor, the GluN2B subunit of the NMDA receptor, or the Gephyrin interacting proteins Collybistin II and GABARAP in neurons not transduced (Control) or transduced with GFE3, RandE3 or GPHN.FingR. Gephyrin expression is reduced in neurons expressing GFE3 (80 ± 3%, P = 0.01, Kruskal-Wallis). No significant change is observed in the steady-state levels of the other proteins (P > 0.8, Kruskal-Wallis; n = 4 replicate blots). (e, f) Staining for the alpha1 subunit of the GABAA receptor (magenta) and Gephyrin (green) in cortical neuron expressing RandE3 (e) or GFE3 (f). Scale bar represents 10 µm.