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. Author manuscript; available in PMC: 2018 Jan 26.
Published in final edited form as: Cell. 2017 Jan 19;168(3):487–502.e15. doi: 10.1016/j.cell.2016.12.022

Figure 2. The tumor microenvironment is remodeled and immune cells transiently proliferate during effective responses.

Figure 2

(A) Statistical Scaffold map of the tumor on day 3. Black nodes are Landmark nodes, representing canonical cell populations identified manually. Other nodes reflect unsupervised clustering of live leukocytes (see Methods). Clusters in red denote populations significantly higher in frequency with effective therapy; blue clusters are significantly lower in frequency. pDC are in the dashed box to maximize space. Colored boxes are populations analyzed in B–E. (B) Expression profile of B cell, (C) cDC, (D) NK cell, or (E) Treg cell clusters expanding with effective therapy (red) versus those decreasing (blue). (F) Scaffold map of Ki67 expression in immune cells in the tumor on day 3. Subsets more proliferative after effective therapy in red. (G) Percent of Ki67+ Treg. (H) Percent of Ki67+ cDC. (I) Scaffold map of the tumor on day 8. (J) Expression profile of macrophage clusters expanding with effective therapy (red) versus those decreasing (blue). (K) Expression profile of ILC cluster increasing with effective therapy. (L) ILC1 frequencies. (M) Expression profile of CD4 T cell or (N) CD8 T cell cluster increasing with effective therapy. (O) Scaffold map of Ki67 expression in immune cells in the tumor on day 8. See also Fig. S2–S4, Tables S2–S3.