Table 2. Effects of treatments with different doses of the chalcone (E)-1-(2-hydroxyphenyl)-3-(4-methylphenyl)-prop-2-en-1-one) (2HMC) on the frequency of micronucleated polychromatic erythrocytes (MNPCE) and polychromatic/normochromatic erythrocyte ratio (PCE/NCE) in bone marrow cells of mice.
| Group | Treatment | MNPCE/2000 PCE | MNPCE reducion (%) | PCE/NCE |
|---|---|---|---|---|
| Control | ||||
| 1 | Negative control (DMSO)1 | 4.2 ± 0.45 | 1.1 ± 0.05 | |
| 2 | Positive control (CPA)2 | 27.2 ± 1.3 | 0.71 ± 0.02 | |
| Genotoxicity and Cytotoxicity | ||||
| 3 | 2HMC 50 mg/kg BW (24 h) | 4.8 ± 0.84 | 0.87 ± 0.04 § | |
| 4 | 2HMC 50 mg/kg BW (120 h) | 5.2 ± 1.30 | 0.77 ± 0.1 § | |
| Co-treatment | ||||
| 5 | 2HMC 25 mg/kg BW (24 h) + CPA | 17 ± 1.58* | 44.3 | 0.78 ± 0.02 |
| 6 | 2HMC 50 mg/kg BW (24 h) + CPA | 15.8 ± 3.03* | 49.6 | 0.71 ± 0.02 |
| Pre-treatment | ||||
| 7 | 2HMC 25 mg/kg BW (120 h) + CPA | 13.2 ± 2.16* | 60.9 | 0.63 ± 0.02 |
| 8 | 2HMC 50 mg/kg BW (120 h) + CPA | 10.8 ± 1.92* | 71.3 | 0.53 ± 0.02 * |
| Post-treatment | ||||
| 9 | CPA + 2HMC 25 mg/kg BW | 27.4 ± 1.81 | 0.71 ± 0.01 | |
| 10 | CPA + 2HMC 50 mg/kg BW | 26.8 ± 0.84 | 0.70 ± 0.01 | |
1Negative control: 0.15 mL dimethylsulfoxide (DMSO) administered orally.
2Positive control: 50 mg/kg BW cyclophosphamide (CPA) diluted in physiological saline and administered ip.
Groups 3 and 4, treated only with 2HMC, were compared to the negative control group.
Groups 5, 6, 7, 8, 9, and 10, co-, pre-, or post-treated were compared to the positive control group.
* Significant compared to the positive control (p < 0.05).
§Significant compared to the negative control (p < 0.05)
The data were analyzed using one-way ANOVA, Tukey’s test, and chi-square test.