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. 2017 Feb 23;2(4):e89044. doi: 10.1172/jci.insight.89044

Figure 2. Genome-wide profiling, qPCR, and Western blot analyses of eWAT.

Figure 2

(A–D) Genome-wide profiling of eWAT with exposure to various temperatures by heat map and volcano analyses (n = 3 samples per group). (E) Left panel: qPCR analysis of Ucp1 expression in eWAT with exposure to various temperatures (n = 6 samples per group). Right panel: qPCR and Western blot analyses of UCP1 protein expression in eWAT (n = 6 samples per group). (F) Quantification of Leptin, Adiponectin, and Resistin mRNA levels in s.c. WAT exposed to various temperatures (n = 6 samples per group). *P < 0.05; **P < 0.01; ***P < 0.001, one-way ANOVA. Box-and-whisker plots show median (line within box), upper and lower quartile (bounds of box), and minimum and maximum values (bars).