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. 2017 Feb 23;2(4):e92340. doi: 10.1172/jci.insight.92340

Figure 5. ZIKV evokes innate inflammatory and antiviral responses in HRvEC.

Figure 5

(A) qRT PCR showing mRNA expression of PRRs (TLR3, RIGI, and MDA5), inflammatory mediators (TNFA, IL1B, IL6, CCL5, and CXCL10), IFNs (IFNA2, IFNB1, and IFNG), and IFN-induced antiviral genes (OAS2, ISG15, and MX1) following ZIKV infection (strain PRVABC59, PR 2015, MOI of 1) (mean ± SEM; n = 3; *P < 0.05, **P < 0.005, ***P < 0.0005; Student’s t test). (B) Western blot showing the expression of TLR3 in human retinal vascular endothelial cell (HRvEC) lysate 24 and 48 hours after ZIKV infection. The bar graph represents densitometry analysis of Western blots using ImageJ with respect to β-actin housekeeping control (mean ± SEM; n = 3; *P < 0.05; Student’s t test).(C) Representative immunofluorescence images showing staining for ISG15 (green), ZIKV (red), and DAPI (blue) 48 hours after ZIKV infection and mock treatment. Original magnification, ×20.