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. 2017 Feb 23;2(4):e87489. doi: 10.1172/jci.insight.87489

Figure 3. Peritumoral adipocytes induce a metabolic remodeling in favor of uncoupled fatty acid oxidation (FAO).

Figure 3

ZR-75-1 cells were cocultivated (C) or not (NC) with adipocytes for 3 days. (A) Quantification of UCP2 mRNA levels (n = 3). (B) UCP2 and inhibitory factor 1 (IF1) expression evaluated by Western blot. α-Tubulin is used as a loading control. (C) Expression of mitochondrial oxidative phosphorylation (OXPHOS) complexes evaluated by Western blot. Actin is shown as a control for equal protein loading. (D) Expression of total and phosphorylated forms of the acetyl-CoA carboxylase (ACC) and AMPK. α-Tubulin is used as a loading control. (B–D) At least 3 experiments were conducted, and representative experiments are shown. (E) Schematic representation of the metabolic remodeling, which takes place in tumor cells in the presence of adipocytes. Bars and error flags represent means ± SEM; statistically significant by Student’s t test, *P < 0.05.