Figure 4. FCGR3A signaling and FCGR hierarchical clustering analysis in response to HIV⁺ patient IgG Abs.

Graphs depict the levels of FCGR3A signaling mediated by HIV-1 Δvpu–infected target cells opsonized with HIV⁺ patient IgG. For FCGR signaling experiments, Abs were diluted 10-fold, starting at a top concentration of 250 μg/ml in a 5-point titration curve. (A and C) The maximum signals achieved at the top concentration of Abs tested (250 μg/ml). (B and D) The area under the signaling curve. (A and B) Comparison of VC vs. CIP. (C and D) Comparison of LTNPs, ECs, and CIPs. The mean of 3 experiments for n = 50 IgG is shown.