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An equal amount of RNA from various human tissues (each a pool of 3 tissue donors) and 2 different human islet preparations was reverse transcribed, and miRNA expression was determined for (A) miR-454-3p, (B) miR-222-3p, (C) miR-144-5p, (D) miR-345-5p, (E) miR-24-3p, and (F) miR-140-5p by quantitative real-time PCR. Cycle 33 (Ct = 30) was arbitrarily set as 1.
