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. 2016 Dec 20;292(6):2089–2100. doi: 10.1074/jbc.M116.757112

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FIGURE 4. — TbPRMT1^ENZ/PRO heteromer is the active form of a major T. brucei PRMT. For the in vitro methylation assay, MBP-RGG substrate was incubated with TbPRMTs in the presence of [³H]AdoMet. Proteins were separated by SDS-PAGE and visualized by fluorography and Coomassie staining. TbPRMT1^ENZ (His-ENZ) and TbPRMT1^PRO (His-PRO) do not exhibit any activity under the selected experimental conditions. The heteromer containing His-TbPRMT1^PRO/ENZ exhibits activity comparable with rat GST-PRMT1. Reactions where MBP-RGG was excluded were used as a control (no substrate). S, substrate; E, enzyme; *, contaminant. The image is representative of two technical replicates.