. 2016 Dec 20;292(6):2089–2100. doi: 10.1074/jbc.M116.757112

TABLE 1.

List of primers

Primer name	Primer sequence
4A	`AGCTTCAATTGCTCGAGGGATCCATGTGGAGCC`
4B	`CCACATGGATCCCTCGAGCAATTGA`
5A	`ATCCGCAGTTCGAGAAGCATCATCACCATCA`
5B	`GGTGATGATGCTTCTCGAACTGCGGATGGCT`
6A	`CCACCATCACTCTAGATAGA`
6B	`GATCTCTATCTAGAGTGATGGTGGTGAT`
13A	`TCGAGGGATCCGGTGGCTCCGGAGGTAGTA`
13B	`GATCTACTACCTCCGGAGCCACCGGATCCC`
qPCR1 TbPRMT1^ENZ 5′	`CGTTCTCACTGCTTTGTTTG`
qPCR1 TbPRMT1^ENZ 3′	`TTTCCGAAGAAGTGGAAGAG`
qPCR1 TbPRMT1^PRO 5′	`GCGTGTATGGGGGTATTTAG`
qPCR1 TbPRMT1^PRO 3′	`AACATGTTCTTGCACACGAC`
qPCR2 TbPRMT1^ENZ 5′	`GGTTTAAGGGCACAATTCGC`
qPCR2 TbPRMT1^ENZ 3′	`ACACCTTTTGTTTCCCGAGG`
qPCR2 TbPRMT1^PRO 5′	`GAAGCAATGTCCTCACAGACT`
qPCR2 TbPRMT1^PRO 3′	`AAGCAGACAAACAGACGCT`
TbPRMT1^ENZ 5′ AflII	`GACTTAAGATGACGGTGGACGCAAATGCCGCC`
TbPRMT1^ENZ BamHI 3′ STOP	`GAGGATCCCTACCGCAGCCGAAAATCCTGG`
BirA STOPdel 5′	`CGCAGAGAAGCTCGAGCTTAAGATGACGGTG`
BirA STOPdel 3′	`CACCGTCATCTTAAGCTCGAGCTTCTCTGCG`
pETDuet BamH1 frame fix 5′	`CATCATCACCACAGCGCAGGATCCATG`
pETDuet BamH1 frame fix 3′	`CATGGATCCTGCGCTGTGGTGATGATG`
TbPRMT1^ENZ-ORF-BamHI-5′	`GTAGGATCCATGACGGTGGACGCAAATG`
TbPRMT1^ENZ -ORF-HindIII-3′	`GTAAAGCTTCTACCGCAGCCGAAAATCCT`
TbPRMT1^ENZ -ORF-NdeI-5′	`GTACATATGATGACGGTGGACGCAAATG`
TbPRMT1^ENZ -ORF-XhoI-3′	`GTACTCGAGCTACCGCAGCCGAAAATCCT`
TbPRMT1^PRO-ORF-BamHI-5′	`GTAGGATCCATGTCACCGAAGAAAAACTCGG`
TbPRMT1^PRO ORF-HindIII-3′	`GTAAAGCTTTCAATACCTTTGGTAGTTGTACGTG`
TbPRMT1^PRO-ORF-NdeI-5′	`GTACATATGATGTCACCGAAGAAAAACTCGG`
TbPRMT1^PRO-ORF-XhoI-3′	`GTACTCGAGTCAATACCTTTGGTAGTTGTACGTG`
G63R TbPRMT1^ENZ 5′	`CTTGATGTTGGTTGCAGGACGGGAATCCTTTC`
G63R TbPRMT1^ENZ 3′	`GAAAGGATTCCCGTCCTGCAACCAACATCAAG`
G106R TbPRMT1^PRO 5′	`GCATTTGGGGTGCAGAATGGGGTTGG`
G106R TbPRMT1^PRO 3′	`CCAACCCCATTCTGCACCCCAAATGC`
E135Q TbPRMT1^ENZ 5′	`TCCTACTCTATCAGTCTATGTTAAACACCG`
E135Q TbPRMT1^ENZ 3′	`CGGTGTTTAACATAGACTGATAGAGTAGGA`
D180Q TbPRMT1^PRO 5′	`GACCATTCTTGATCAATCAGCCACTGCTAGAAGAGGC`
D180Q TbPRMT1^PRO 3′	`GCCTCTTCTAGCAGTGGCTGATTGATCAAGAATGGTC`