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. 2016 Dec 8;292(6):2101–2109. doi: 10.1074/jbc.M116.764712

FIGURE 2.

FIGURE 2.

Unlike POMGNT1, POMGNT2 exhibits acceptor selectivity. A and B, radioactive assay of POMGNT1 and POMGNT2 activity with Man341 (A), an M1 acceptor, and Man379 (B), an M3 acceptor. Transfer is measured in background-corrected dpm. Error bars represent S.E. of three replicates. C and D, FTMS spectra verifying POMGNT1-extended Man341 (1.16-ppm mass accuracy) (C) and POMGNT1-extended Man379 (2.53-ppm mass accuracy) (D). The green circle represents a mannose, and the blue square represents an N-acetylglucosamine (38). E and F, FTMS spectra verifying POMGNT2 Man341 product (1.11-ppm mass accuracy) (E) and POMGNT2-extended Man379 (1.11-ppm mass accuracy) (F). The green circle represents a mannose, and the blue square represents an N-acetylglucosamine (38).