A conserved consensus sequence for POMGNT2 activity.
A, sequence alignment of 21-mer sequences of α-DG centered on known O-mannose sites from human and rabbit (20, 21) excluding Thr-317 and Thr-379. The logo was made using Berkeley's WebLogo program. B, sequence alignment of 21-mer sequences of α-DG centered on human sites Thr-317 and Thr-379 from all Ensembl vertebrates with orthologues of DAG1, POMGNT1 or POMGNT2, and FKTN or B4GAT1 (total of 59 species; see “Experimental Procedures” for a complete list). The logo was made using Berkeley's WebLogo program.