FIGURE 3.
Nuclear c-FLIPL crucial for β-catenin transactivation. A, design for the cytoplasmic localization of c-FLIPL. The nuclear export signal (NES) sequence from MAPKK was inserted into the N-terminal of c-FLIPL. ▵NLS-FLIPL is a truncated mutant of c-FLIPL lacking NLS at the C-terminal. All constructs are tagged with c-Myc tag in the N terminus. B, 293T cells were co-transfected with TOPFLASH or FOPFLASH reporter gene constructs and various FLIPL expression vectors. After 24 h transfection, cells lysates were analyzed by luciferase assay. The fold-change of the luciferase activity was shown as mean ± S.D.(n = 3). C, 293T cells were transfected with various FLIPL constructs (0.2 μg) and prepared for the cytoplasmic and nuclear fractions to detect β-catenin expression by Western blotting. Tubulin as a control for equal protein loading.