FIGURE 6.
c-FLIPL-driven cell growth related with TIP49 expression. A, colony formation ability of A549 cells with stable expression of c-FLIPL versus control vector. Cell growth for 3 weeks is shown in representative pictures. The number of colony formations was counted for quantitation and the protein level of c-FLIPL was detected as shown at the bottom. B, the tumor growth curve of con-A549 and FLIPL-A549 (n = 4 each) in the mouse model of tumor implantation. Data were presented as mean ± S.E., *, p < 0.05. C, the proliferation assay by RTCA in A549 stable cells. A549 cells were treated with siRNAs for 24 h, and then the counted cells at the optimal number (1 × 104 cells/well) were seeded in E-plates. Impedance measurements were performed in a time-resolved manner observed for a period of 72 h. D, growth rate was calculated from 6 to 36 h according to RTCA data. E, doubling time for the population was shown in software analysis according the growth curves. F, A549 stable cells were seeded in the E-plate and attached to the wall for 4 h, then FH535 (20 nm) was added to each well. Impedance measurements were performed in a time-resolved manner observed for 32 h.