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. 2016 Dec 27;292(6):2191–2202. doi: 10.1074/jbc.M116.736306

FIGURE 5.

FIGURE 5.

The role of LRAP35a in DC migration in 2D and 3D environments. A, RT-PCR analysis for the expression of Lurap1 encoding LRAP35a in BM-derived DCs transfected with siRNA or control oligonucleotide. B, effect of LRAP35a knockdown on the migration speed of Dock8+/− BM-derived DCs on a 2D surface (left panel) or in 3D collagen gels (right panel). As controls, Dock8+/− and Dock8−/− BM-derived DCs transfected with control oligonucleotide were analyzed simultaneously. At least 30 cells were analyzed per category. The central lines and error bars on the scatterplots represent the mean and S.D., respectively. **, p < 0.01 (two-tailed Student's t test). Data were collected from three separate experiments. C, effect of overexpression of the GFP fusion LRAP35a N mutant on the migration speed of Dock8+/− BM-derived DCs in 3D collagen gels. As controls, Dock8+/− and Dock8−/− BM-derived DCs transfected with GFP alone were simultaneously analyzed. At least 20 cells were analyzed per category. The central lines and error bars on the scatterplots represent the mean and S.D., respectively. **, p < 0.01 (two-tailed Student's t test). Data were collected from three separate experiments.