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. 2016 Dec 29;292(6):2237–2254. doi: 10.1074/jbc.M116.764233

TABLE 2.

List of primers for the MNase protection assay

M, amplicon midpoint, relative to CISH TSS (bp).

M Forward primer (5′–3′) Reverse Primer (5′-3′)
−453 CACACGCCGACAGACCTC GGGAAGGGCCCTCTTATCTC
−438 GCTCGCAGGGAGGACAA GGGAAGGGCCCTCTTATCT
−376 TTCCACCGCGAGATAAGAGG GGGTCGGGGAAGTTAAGGAG
−344 GACGCAGAATGCCAGAAGG GGCAGGTCGGAGAGATCAGT
−322 GGGCCCCTCCTTAACTTCC GGCAGGTCGGAGAGATCAGT
−284 CCACTGATCTCTCCGACCT CAATAGCAGCGCGTGGA
−256 GACCTGCCCCTCTGGGTA AATCGCGACGCTGAAGGT
−214 GTCCACGCGCTGCTATTG GACAGATTTCCAAGAACTTTCCA
−174 GTCGCGATTGGTCAGCTC GGGCCCTGAGCAGTGAAA
−157 TTCCTGGAAAGTTCTTGGAAATC GGCCCTGAGCAGTGAAA
−54 TCAGCCCGCGGTTCTA TTTGTCTGCCGCGTTCC
−42 CCCGCGGTTCTAGGAAGA GGCGAGCTGCTGCCTAAT
−29 TCCGGGAAGGGCTGGAA GGCGAGCTGCTGCCTAATC
+20 AAAGGATTAGGCAGCAGCTC GACAGGGACTGAGAGGCAGT
+76 CACTGCCTCTCAGTCCCT AGCGCGTGCTGGGTA
+90 TGCCTCTCAGTCCCTGTCC GGCTGGAGGGAACCAGTG
+164 CCCACTGGTTCCCTCCAG AGAGAGCCGCGCTTACCC
+208 GACATGGTCCTCTGCGTTCAG GTGGTGGCCGGGAAGG
+225 ACATGGTCCTCTGCGTTCAG TAGCACGAAGCCCCTGTTCT
+249 GTAAGCGCGGCTCTCTG TCAAGCCCTCCCAATGC
+295 AGAACAGGGGCTTCGTGCTA CTTGCTCCTGAGGTCCGTCT
+324 GCATTGGGAGGGCTTGA GGAGTGGGAGTGTCACAG
+384 GCTGTGACACTCCCACTC CCGGTTTCCCAATCCACA
+412 CCCCCACACACTTACCTCAA CCACCCTGTGAGTTCCTCCT