FIGURE 6.

TRC8 decreases trafficking and function of hERG. A, HEK293 cells were transfected with hERG and the indicated TRC8 construct or vector control and examined for hERG kinetics by pulse-chase as in Fig. 2A. Data points and lines representing the averages are shown. B, HEK293 cells were transfected with hERG, GFP, and either TRC8 or vector control. Voltage-response curves from patch clamp measurements as in Fig. 1E are shown. C, cells were transfected as in A with the indicated HA-tagged TRC8 construct, and hERG was immunoprecipitated (IP). Co-precipitating TRC8 was detected by immunoblot using specific antibodies against TRC8 and the HA tag, and Hsp70 was detected as a control. Quantitation of TRC8 was adjusted for the total amount of immunoprecipitated hERG and plotted relative to the amounts in the control. *, p < 0.05; **, p < 0.01 relative to controls.