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Basal and activin A-stimulated Fshb expression were abolished in cKO pituitary cells. Primary pituitary cells were prepared from adult male (A) or female (B) control or cKO mice and treated with 1 nm activin A (red) or with vehicle (black). Fshb mRNA levels were measured by RT-qPCR. Bars represent the means (+ S.E.) of three independent experiments. Bars with different symbols (a, b, c, and d) differ significantly.
