FIGURE 4.

cKO males and females are FSH deficient. A, B, E, and F, FSH (A and B) and LH (E and F) levels in mouse serum were measured by multiplex ELISA. Individual data points are shown (n = 12 or 14 for control and cKO, respectively) as are the group means (horizontal lines). C and D, pituitary FSH content was assessed by RIA (n = 8 or 5 for control and cKO males; n = 11 or 7 for control and cKO females). All samples from cKO females were below the detection limit of the assay. G and H, pituitary LH content was measured by ELISA. The data were analyzed by Student's t test in each panel.