. 2016 Dec 19;292(6):2301–2314. doi: 10.1074/jbc.M116.759167

TABLE 2.

Genotyping and qPCR primers

	5′ → 3′
Genotyping
Smad3
Forward	`CTCCAGATCGTGGGCATACAGC`
Reverse	`GGTCACAGGGTCCTCTGTGCC`
Recombined	`TCGTCGATCGACCTCGAATAAC`
Smad4
Forward	`GGGCAGCGTAGCATATAAGA`
Reverse	`GACCCAAACGTCACCTTCAG`
Recombined	`AAGAGCCACAGGTCAAGCAG`
GRIC
Forward	`GGACATGTTCAGGGATCGCCAGGC`
Reverse	`GCATAACCAGTGAAACAGCATTGCTG`
ROSA26 eYFP
Forward	`AAAGTCGCTCTGAGTTGTTAT`
WT reverse	`GCGAAGAGTTTGTCCTCAACC`
eYFP reverse	`GGAGCGGGAGAAATGGATATG`

qPCR
Smad3 exons 3/4
Forward	`CATTCCATTCCCGAGAACAC`
Reverse	`ATGCTGTGGTTCATCTGGTG`
Smad3 exons 8/9
Forward	`CATCCGTATGAGCTTCGTCA`
Reverse	`CATCTGGGTGAGGACCTTGT`
Smad4
Forward	`TCACAATGAGCTTGCATTCC`
Reverse	`CCATCCACAGTCACAACAGG`
Fshb
Forward	`GTGCGGGCTACTGCTACACT`
Reverse	`CAGGCAATCTTACGGTCTCG`
Rpl19
Forward	`CGGGAATCCAAGAAGATTGA`
Reverse	`TTCAGCTTGTGGATGTGCTC`
Gnrhr
Forward	`CACGGGTTTAGGAAAGCAAA`
Reverse	`TTCGCTACCTCCTTTGTCGT`
Lhb
Forward	`AGCAGCCGGCAGTACTCGGA`
Reverse	`ACTGTGCCGGCCTGTCAACG`
Cga
Forward	`TCCCTCAAAAAGTCCAQGAGC`
Reverse	`GAAGAGAATGAAGAATATGCAG`