FIGURE 1.

Developmental defects in tsu3994 mutant embryos. A and B, Mt embryos showing reduced pigmentation and shorter caudal fin at 36 hpf (A) and additionally hemorrhage (indicated by arrowheads) at 48 hpf (B). Wild-type siblings (WT sib) from the same batch served as control. C, O-dianisidine staining detected leaking red blood cells in the head, eyes, and trunk (indicated by arrowheads) in mutant embryos at 48 hpf. D, vascular defects in mutants in Tg(fli1a:EGFP) transgenic background at 48 hpf. Some intracerebral vessels (top panels, dorsal views) and intersegmental vessels (middle panels, lateral views) were disappeared or disconnected (indicated by arrowheads). The bottom panels show enlarged parts. MsV, mesencephalic vein; DA, dorsal aorta; PCV, posterior caudal vein.