FIGURE 6.

The hydrophobic property of L1246 is required for Gbf1 translocation to the Golgi apparatus. A, illustration of predicted hydrophobic face of the corresponding α-helix. Ley → Arg substitution would disrupt the hydrophobic face (dashed lines). B, Flag-Gbf1 with different residues at the position 1246 showed different locations in HeLa cells. The HeLa cells transfected with different expression constructs were treated for 12 h with 10 μm GCA before harvest for immunostaining with Flag antibody. Representative confocal images are shown on the left. The intracellular location is summarized in the table. The ERGIC/Golgi location was reflected by enriched signals in the perinuclear region.