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. 2016 Dec 21;292(6):2315–2327. doi: 10.1074/jbc.M116.767608

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FIGURE 8. — Gbf1(L1246R) mutant protein fails to recruit β-COP to the Golgi apparatus. Flag-tagged Gbf1(M830L) or Gbf1(M830L;L1246R) was transfected into HeLa cells. The transfected cells were incubated for 24 h, and then DMSO or GCA was added. The cells were harvested for immunodetection of Flag-Gbf1 and endogenous β-COP 12 h post DMSO (A–C) or GCA treatment (D–F). Regardless of GCA, expression of Gbf1(M830L) promoted recruitment of β-COP to the perinuclear Golgi region (B and E), whereas Gbf1(M830L;L1246R) expression had no effect (C and F). Arrows in (B and E) indicated cells with low level Gbf1(M830L) expression and showing less amount of enriched β-COP compared with cells with high-level Gbf1(M830L) expression.