FIGURE 7.

Ultrasound-targeted TRAF3IP2, p65, or JNK1 AS-ODN attenuated I/R-induced myocardial injury. A, details of the I/R protocol are shown. B, TRAF3IP2 AS-ODN is highly effective in reducing I/R-induced myocardial injury. C56BL/6 mice underwent 30 min ischemia/24 h reperfusion. At the initiation of reperfusion, PESDA-bound TRAF3IP2, p65, or JNK1 AS-ODN (AS) were delivered arterially into left ventricle for 10 min with simultaneous application of ultrasound. 24 h later, animals were sacrificed and infarct size quantified. Arrows indicate the infarcted region. Although representative images are shown at the top, results from 6 to 8 mice/group are summarized on the bottom. C–E, administration of TRAF3IP2, p65, or JNK1 AS-ODN inhibits their target protein expression in the heart. Following delivery of AS-ODN, expression of respective target proteins was analyzed by immunoblotting. Although a representative immunoblot is shown on the left, densitometric analysis of immunoreactive bands from 5 to 6 animals is summarized on the right. B–E, *, p < 0.01 versus sham; †, p < 0.05 versus I/R ± control; ††, p < 0.01 versus I/R + PESDA (n = 6–8/group).