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. 2017 Jan 4;292(6):2345–2358. doi: 10.1074/jbc.M116.764522

TABLE 3.

Sequence of phosphorothioated AS-ODN against p65, JNK1, and TRAF3IP2

Target ODN ODN sequencea Length
p65 AS 5′-G*A*A*A*C*A*G*A*T*C*G*T*C*C*A*T*G*G*T-3′ 19
Scrambled 5′-G*G*A*A*C*A*G*T*T*C*G*T*C*T*A*T*G*G*C-3′ 19
JNK1 AS 5′-T*G*T*T*G*T*C*A*C*G*T*T*T*A*C*T*T*C*T*G-3 20
Control 5′-C*C*T*T*C*C*C*T*G*A*A*G*G*T*T*C*C*T*C*C-3′ 20
TRAF3IP2 Oligo#1-AS 5′-G*C*C*A*C*A*T*A*C*T*C*T*T*C*C*T*C*C*C*T-3′ molecular mass: 6229.0 Da; 3′ end position: 2023 20
Oligo#2-AS 5′-G*C*C*A*C*A*T*A*C*T*C*T*T*C*C*T*C*C*C*T*G-3′ molecular mass: 6574.3 Da; 3′ end position: 2022 21
Oligo#3-AS 5′-G*C*C*A*C*A*T*A*C*T*C*T*T*C*C*T*C*C*C-3′ molecular mass: 5908.8 Da; 3′ end position: 2024 19
Oligo#4-AS 5′-A*G*C*C*A*C*A*T*A*C*T*C*T*T*C*C*T*C*C*C*T-3′ molecular mass: 6558.3 Da; 3′ end position: 2023 21
Oligo#4scrambled 5′-A*C*T*C*C*A*T*C*C*G*T*C*C*T*A*C*T*C*A*T*C-3′ molecular mass 6558.3 Da 21

a p65, the p65 subunit of NF-κB; JNK1, c-Jun N-terminal kinase 1; TRAF3IP2, TRAF3 interacting protein 2; asterisk represents phosphorothioated.