FIGURE 4.
C. reinhardtii ADHE is located in the chloroplast stroma. A, organelle fractionation from strain 10-6C. Ce, cell extract; Cp, chloroplast fraction; Mt, mitochondrial fraction. Proteins were separated on SDS-PAGE (5–12% acrylamide gradient). Immunoblots show the distribution in isolated organelles of LHCs, subunit β of the mitochondrial F0F1-ATPase (ATP2), and ADHE. B, two-dimensional resolution of proteins in a stromal fraction. Proteins were subjected to BN (3–12%)/SDS-PAGE (10%) and further transferred to nitrocellulose for immunodetection. The blot was first probed for ADHE and then for PFL. ★, position of the ADHE dimer; ▾, position of the PFL dimer. C, EM images of a stromal protein fraction using uranyl acetate as negative stain.