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. 2017 Jan 5;292(6):2411–2421. doi: 10.1074/jbc.M116.762807

FIGURE 1.

FIGURE 1.

Effect of AZA on the expression of junctional proteins. A, A549 cells were treated with dimethyl sulfoxide (DMSO) vehicle (0 μm) or AZA for 24 h at the indicated concentration. Cell lysates were immunoblotted with anti-claudin-1, anti-claudin-2, anti-occludin, anti-E-cadherin, and anti-β-actin antibodies. B, PVDF membrane was stained with CBB after immunoblotting. C, expression levels of claudin-1, claudin-2, occludin, and E-cadherin are represented as percentage of the values in 0 μm. D, cells were treated with DMSO vehicle (control) or AZA for 6 h. After isolation of total RNA and reverse transcription, quantitative real time PCR was performed using primers for claudin-1, claudin-2, occludin, E-cadherin, and GAPDH. β-Actin served as an internal control. The expression levels of mRNA are represented as percentage of the values in the control cells. n = 3–4. **, p < 0.01 compared with control or 0 μm. NS, p > 0.05.