TABLE 1.

Primers for PCR amplification

Name	Direction	Sequence
Claudin-1	Forward	5′-ATGAGGATGGCTGTCATTGG-3′
Claudin-1	Reverse	5′-ATTGACTGGGGTCATAGGGT-3′
Claudin-2	Forward	5′-ATTGTGACAGCAGTTGGCTT-3′
Claudin-2	Reverse	5′-CTATAGATGTCACACTGGGTGATG-3′
Occludin	Forward	5′-TTTGTGGGACAAGGAACACA-3′
Occludin	Reverse	5′-TCATTCACTTTGCCATTGGA-3′
E-cadherin	Forward	5′-ACCCCCTGTTGGTGTCTTT-3′
E-cadherin	Reverse	5′-TTCGGGCTTGTTGTCATTCT-3′
GAPDH	Forward	5′-GCCATCAATGACCCCTTCATT-3′
GAPDH	Reverse	5′-TCTCGCTCCTGGAAGATGG-3′
β-Actin	Forward	5′-CCTGAGGCACTCTTCCAGCCTT-3′
β-Actin	Reverse	5′-TGCGGATGTCCACGTCACACTTC-3′
PTEN (Met)	Forward	5′-GTTTGGGGATTTTTTTTTCGC-3′
PTEN (Met)	Reverse	5′-AACCCTTCCTACGCCGCG-3′
PTEN (Un-met)	Forward	5′-TATTAGTTTGGGGATTTTTTTTTTGT-3′
PTEN (Un-met)	Reverse	5′-CCCAACCCTTCCTACACCACA-3′
miR-15a	Forward	5′-TAGCAGCACATAATGGTTTGTG-3′
miR-15b	Forward	5′-TAGCAGCACATCATGGTTTACA-3′
miR-16	Forward	5′-TAGCAGCACGTAAATATTGGCG-3′
miR-195	Forward	5′-TAGCAGCACAGAAATATTGGC-3′
miR-424	Forward	5′-CAGCAGCAATTCATGTTTTGAA-3′
miR-497	Forward	5′-CAGCAGCACACTGTGGTTTGT-3′