FIGURE 3.

Specific inhibition of HDAC1 blocks rhodopsin expression. A–H, immunofluorescence microscopy of PN1 retina explants cultured for 96 h with DMSO (control; A, E, and G) or treated with different concentrations of CAS 193551-00-7 (HDAC1i): 0.1 (B), 0.5 (C), and 1.0 μm (D, F, and H). Cryosections were stained with anti-RHO (green) (A–H), anti-H4K12ac (red) (A–D), anti-H3K9ac (red) (E and F), and anti-H3K27ac (red) (G and H) antibodies, and nuclei were counterstained with Hoechst 33358 (blue). Image quantification of immunofluorescence intensity was performed for three biological replicates with three technical replicates for each sample and normalized to control ±S.E. I, rhodopsin expression. J, H4K12ac levels. K, H3K9ac levels. L, H3K27ac levels. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Error bars represent S.E.