FIGURE 6.

Selective inhibition of HDAC1 increases global level of H3K9ac and H4K12ac but not H3K27ac. Western blots of samples isolated from mouse PN1 retina explants cultured for 96 h with DMSO (control) or treated with 1.0 μm CAS 193551-00-7 (HDAC1i), 16 nm apicidin (HDAC3i), 54 nm romidepsin (HDAC1,2i), 5 μm entinostat (HDAC class I inhibitor), 36 μm MC-1568 (HDAC class IIa inhibitor), or 5 μm sodium butyrate (pan-HDAC inhibitor) are shown. Blots were probed with antibodies against H4K12ac (A), H3K9ac (B), and H3K27ac (C). Coomassie staining of core histones was used as a loading control (D). Band intensity quantification was performed for three biological replicates for each histone acetylation mark and normalized to control ±S.E. E, the dashed rectangle in Coomassie staining of core histones indicates the position where Western blots were spliced. **, p < 0.01. Error bars represent S.E.