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. 2016 Dec 27;292(6):2422–2440. doi: 10.1074/jbc.M116.756643

TABLE 2.

PCR primers used in this work

Gene Sequence Fragment size
bp
cdna_Apaf1-Fa GTA CAC CCC CTG AAA AGC AA 108
cdna_Apaf1-Ra CAG GGT GGG TCA CCA TCT AT
cdna_Bcl2-Fa AGC CCG TGT TTG TAA TGG AG 476
cdna_Bcl2-Ra CAC AGC CTT GAT TTT GCT GA
cdna_Caspase3-Fa AGG GGT CAT TTA TGG GAC AAA 422
cdna_Caspase3-Ra TAC ACG GGA TCT GTT TCT TTG
cdna_Caspase9-Fa CAG GCC CGT GGA CAT TGG TT 438
cdna_Caspase9-Ra CAG CCG CTC CCG TTG AAG ATA
cdna Ccnd1 Qiagen catalog number QT00154595 108
cdna Crx Qiagen catalog number QT00115402 127
cdna_Dll1-F TCG ACT CCT TCA GCC TGC 233
cdna_Dll1-R GCT ACT GTG AAG GTC CTG AGA C
cdna_Foxn4-F GTG AGA TCT ACA GCT TCA TGA AGG 275
cdna_Foxn4-R TGA GAT GAG CTT GTC CAA CTC C
cdna GAPDH Qiagen catalog number QT01658692 144
cdna_Gnat2-F ACC ATG CCT CCT GAG TTG 187
cdna_Gnat2-R TGA CTC TGG ATC GAA GCA C
cdna_Hes1-F TTC CAA GCT AGA GAA GGC AGA C 191
cdna_Hes1-R GCA CCT CGG TGT TAA CGC
cdna_Hes5-F AAG CTG CTG CTG GAG CAG 229
cdna_Hes5-R GCA GCT TCA TCT GCG TGT C
cdna Isl1 Qiagen catalog number QT01048691 79
cdna_NeuroD1-F CCT GTG ACC TTT CCC ATG C 219
cdna_NeuroD1-R AGA AGT GCT AAG GCA ACG C
cdna_Notch1-F GCT CCG AGG AGA TCA ACG AG 268
cdna_Notch1-R TTG ACA TCA CCC TCA CAC CG
cdna_Nrl-F GTG CCT CCT TCA CCC ACC TTC AGT GA 361
cdna_Nrl-R GCG TGC GGC GCC TCT GCT TCA GCC G
cdna_Otx2-F TCG CCA CCT CTA CTT TGA TAG 85
cdna_Otx2-R AGC CGC ATT GGA CGT TAG
cdna_Pax6-F TTT AAC CAA GGG CGG TGA GCA G 475
cdna_Pax6-R TCT CGG ATT TCC CAA GCA AAG ATG
cdna_Pde6b-F CTG ACG AGT ATG AGG CCA AAG 204
cdna_Pde6b- R TAG GCA GAG TCC GTA TGC AGT
cdna_Pde6c-F CCT TAT GTG GTC AGC CAA TAA AG 210
cdna_Pde6c-R CCA TCT GGA GTC TTT GGT CC
cdna_Rcvrn-F GCA GCT TCG ATG CCA ACA G 66
cdna_Rcvrn-R TCA TGT GCA GAG CAA TCA CGT A
cdna_Rgr-F TTG TGT GGA TGT CAT CTG C 184
cdna_Rgr-R GAA GTG TGT GTG ATG AAC AGG
cdna_Rho-F CTT CTC CAA CGT CAC AGG CGT 346
cdna_Rho-R GGA CCA CAG GGC GAT TTC AC
cdna_Sag-F GCC ATG AGT GTC CTC ACC 255
cdna_Sag-R GGC ATG CTG CAC TTT CC
cdna_Kcnv2-F CTT CTC TGC AGC TGT CTA CTC G 226
cdna_Kcnv2-R GTA ATC GGA GAA CTT GTT GTA GAG G
cdna Opn1w Qiagen catalog number QT00163723 1221
cdna_Opn1sw-F CAG CAT CCG CTT CAA CTC CAA 229
cdna_Opn1sw-R GCA GAT GAG GGA AAG AGG AAT GA
cdna PKCα Qiagen catalog number QT00146384 101
cdna Rom1 Qiagen catalog number QT00172165 132
cdna_Samd11-F CTT TCT GGC TGT GGC GAG 173
cdna_Samd11-R GCC ATG TAG AAG ACA CGG C
cdna Tfap2b Qiagen catalog number QT00135478 83
cdna_Vsx2-F1 ACG GAG CTC CCA GAA GAC 176
cdna_Vsx2-R1 CCA TCC TTG GCA GAC TTG
chip_Ccnd1-F TTA GAA TAA AGC GGT TCC ACC 284
chip_Ccnd1-R TTC GGA GCT ACA GTG GAA TC
chip_Crx-F GCT CAG GTT GGC CTC AGA C 179
chip_Crx-R CCA CAC TAG TGC GAG ACC TGA G
chip_Foxn4-F GGG AGA GAC TAC TAC CTT TGA CTT CTC 248
chip_Foxn4-R CCC TAC GTG CTC TAT AGA GAA TAA TAG C
chip_Gapdh-F CCT CAA CTT TTC CGC AGC 331
chip_Gapdh-R CCT CCT CCC TCT CTT TGG
chip_Gnat2-F TTT GAA CTG AGA GTG CAT TTC TAA C 185
chip_Gnat2-R GAC TCC TAT TCT GCT ACT GCT CTG
chip_Hes1-F GCC TGG CCA CAA AAG AAA TA 238
chip_Hes1-R CCC AAA CTT TCT TTC CCA CA
chip_Notch1-F CTA TAG GCA TCA GGA GGA TTG AG 204
chip_Notch1-R CCG TGG AAC GTC TAG ACT CG
chip_Nrl-F TGC CTT GGA GAG CCT AGC 160
chip_Nrl-R AAG CAG GAG TCC TAT CCA CTT C
chip_Rho-F CCA CTT CAG ACT CTA GGC CTC 114
chip_Rho-R GTG TTG GCT CAG TGA GAC AAG
chip_Tfap2b-F CAT CAT TGT AGA CTT ACT TCT GGA GC 73
chip_Tfap2b-R TTC TTT AAG GGC TTG GCT ACC
chip_Vsx2-F1 CAG AGC CCA CCA CAC TTG 190
chip_Vsx2-R1 GGT GTC TAA TGC TCT GGA GC
chip_Rho_F1 GGA ATT CCC AGA GGA CTC TG 287
chip_Rho_R1 CTC TTC GTA GAC AGA GAC C
chip_Rho_F2 TGA CCT CTT CAT GGT CTT CG 134
chip_Rho_R2 CAG TCT CTC TGC TCA TAC CTC C
chip_Rho_F3 CCA GGA GTG AGC TCT AGC TT 117
chip_Rho_R3 GGC AGT GAG ATG TAC AAG TTT AC
chip_Vsx2_F2 AAC AGT CCC TAT GCA CCT GTA TC 139
chip_Vsx2_R2 CAC ACA GTA CTT GGA GTT TGG G
chip_Vsx2_F3 CCA TAC AGA TGT TGT TCC TGC 206
chip_Vsx2_R3 CCT TAC AAC AGG AGA GAA CCC

a Ref. 46.