TABLE 1.

Details of the top seven sequence alignments with the IQ motif of UBE3B using Phyre²

The alignment coverage, confidence, and % ID for the IQ motif are shown. The alignment coverage gives the percentage of alignment between the query sequence and the template; the confidence represents the probability (from 0 to 100) that the match between the query sequence and the template is a true homology, and the % ID is the likelihood the model is accurate. For extremely high accuracy models, the % ID should be above 30–40%; however, if the confidence is high, even very low % ID (<15%) can be very useful.

Rank	Template	Alignment coverage	Confidence	% ID
		%
1	Myosin-5a	90	86.7	32
2	Myosin Va	83	79.0	31
3	Unconventional myosin-1C	73	77.4	39
4	Myosin	66	77.0	24
5	Smooth muscle myosin heavy chain	86	74.9	19
6	Myosin heavy chain, muscle	66	74.3	30
7	Myosin heavy chain	86	74.3	30