TABLE 2.

Details of the top seven sequence alignments with the HECT domain of UBE3B using Phyre²

The alignment coverage, confidence, and % ID for the HECT domain are shown. The alignment coverage gives the percentage of alignment between the query sequence and the template; the confidence represents the probability (from 0 to 100) that the match between the query sequence and the template is a true homology, and the % ID is the likelihood the model is accurate. For extremely high accuracy models, the % ID should be above 30–40%; however, if the confidence is high, even very low % ID (<15%) can be very useful.

Rank	Template	Alignment coverage	Confidence	% ID
		%
1	Rsp5	97	100.0	34
2	Huwe1	97	100.0	37
3	HECT, E3 ligase	97	100.0	34
4	Nedd4-like	96	100.0	36
5	Smad	96	100.0	33
6	HECT, E3 ligase	97	100.0	30
7	Ubr5	38	100.0	30