TABLE 3.

Oligodeoxynucleotides used in this study

F is forward; R is reverse.

Oligonucleotide name	Sequence (5′–3′)a
UBE3B-GFP-F	ATTCTAGACACCATGTTCACCCTGTCTCAGACCTCG
UBE3B-GFP-R	TAGCTCTAGAATGGAGAGTTCAAAGCCCGTGTTCATGC
UBE3BΔHECT-GFP-R	ATCGGATCCAATCCCCACTGGTTGTCTTGAACAGATT
UBE3B(C1036A)-F	TGCCCACCTCCTCCACCGCCTTCAACCTGCTCAAGC
UBE3B(C1036A)-R	GCTTGAGCAGGTTGAAGGCGGTGGAGGAGGTGGGCA
HA-UBE3A-F	CACCATGTACCCATACGATGTTCCAGATTACGCTGAGAAGCTG
HA-UBE3A-R	TTACAGCATGCCAAATCCTTTGGCATACGTGATGG
HA-UBE3B-F	CACCATGTACCCATACGATGTTCCAGATTACGCTTTCACCCTGTCTCAGACC
UBE3B-R	CTAGGAGAGTTCAAAGCCCGTGTTCATGCTGATGGCG
UBE3B-F	CACCATGTTCACCCTGTCTCAGACCTCGAGAGCATGG
UBE3BΔHECT-R	CTAGGAGAGTTCAAAGCCCGTGTTCATGCTGATGGCG
UBE3B-HA-R	CTAAGCGTAATCTGGAACATCGTATGGGTAGGAGAGTTCAAAGCCCGTG
Ubiquitin-F	CACCCAGATTTTCGTGAAAACCCTTACG
Ubiquitin-R	TTAACCACCACGAAGTCTCAACACAAGATG
UBE3B(ΔIQ)-F	CACCATGGCAGATGACCCTGAGTCCACTAAAAGAAGTGC

^a The bold bases indicate the mutated bases.