TABLE 4.
Vectors developed for and used in this study
| Plasmid name | Insert description | Parental vector |
|---|---|---|
| pENTR-UBE3B | UBE3B | pENTR/d-TOPO |
| pENTR-HA-UBE3B | HA-UBE3B | pENTR/d-TOPO |
| pENTR-UBE3B-HA | UBE3B-HA | pENTR/d-TOPO |
| pENTR-HA-UBE3BΔHECT | HA-UBE3BΔHECT | pENTR/d-TOPO |
| pENTR-HA-UBE3B(C1036A) | HA-UBE3B (C1036A) | pENTR/d-TOPO |
| pENTR-HA-UBE3A | HA-UBE3A | pENTR/d-TOPO |
| pENTR-UBE3BΔIQ-HA | UBE3BΔIQ-HA | pENTR/d-TOPO |
| pCT-CMV-UBE3B-copGFP-Puro | UBE3B-copGFP | pCYTO-CMV-MCS-copGFP-EF1-Puro |
| pCT-CMV-UBE3BΔHECT-copGFP-Puro | UBE3BΔHECT-copGFP | pCYTO-CMV-MCS-copGFP-EF1-Puro |
| pCT-CMV-UBE3BΔHECT-copGFP-Puro | UBE3B (C1036A)-copGFP | pCYTO-CMV-MCS-copGFP-EF1-Puro |
| pLVX-HA-UBE3B-IRES-Puro | HA-UBE3B | pLVX-GW-IRES-Puro |
| pLVX-UBE3B-HA-IRES-Puro | UBE3B-HA | pLVX-GW-IRES-Puro |
| pLVX-HA-UBE3BΔHECT-IRES-Puro | HA-UBE3BΔHECT | pLVX-GW-IRES-Puro |
| pLVX-HA-UBE3B(C1036A)-IRES-Puro | HA-UBE3B (C1036A) | pLVX-GW-IRES-Puro |
| pLVX-HA-UBE3A-IRES-Puro | HA-UBE3A | pLVX-GW-IRES-Puro |
| pLVX-UBE3BΔIQ-HA-IRES-Puro | UBE3BΔIQ-HA | pLVX-GW-IRES-Puro |
| pLVX-BirA-UBE3B-Puro | BirA-UBE3B | pLVX-GW-IRES-Puro |
| pLVX-UBE3B-BirA-Puro | UBE3B-BirA | pLVX-GW-IRES-Puro |