TABLE 5.
Cell lines developed and used in this study
| Cell line name | Cell line description | Growth mediaa |
|---|---|---|
| LN428 | Human glioblastoma tumor cell line | Media 1 |
| LN428/UBE3B-copGFP | LN428 cells expressing UBE3B-copGFP | Media 2 |
| LN428/UBE3BΔHECT-copGFP | LN428 cells expressing UBE3BΔHECT-copGFP | Media 2 |
| LN428/UBE3B(C1036A)-copGFP | LN428 cells expressing UBE3B (C1036A)-copGFP | Media 2 |
| LN428/HA-UBE3B | LN428 cells expressing HA-UBE3B | Media 2 |
| LN428/UBE3B-HA | LN428 cells expressing UBE3B-HA | Media 2 |
| LN428/HA-UBE3BΔHECT | LN428 cells expressing HA-UBE3BΔHECT | Media 2 |
| LN428/HA-UBE3B(C1036A) | LN428 cells expressing HA-UBE3B (C1036A) | Media 2 |
| LN428/HA-UBE3A | LN428 cells expressing HA-UBE3A | Media 2 |
| LN428/BirA-UBE3B | LN428 cells expressing BirA-UBE3B | Media 2 |
| LN428/UBE3B-BirA | LN428 cells expressing UBE3B-BirA | Media 2 |
| 293FT | A sub-clone of human embryonic kidney cells 293 | Media 3 |
a Media 1 is α-minimal essential medium with 10% heat-inactivated FBS, 5 μg/ml gentamicin, 80 μg of penicillin, 80 μg of streptomycin, 0.32 μg of amphotericin/ml, 2 mm l-glutamine. Media 2 is Media 1 supplemented with puromycin (1.0 μg/ml). Media 3 is DMEM with 10% heat-inactivated FBS, 80 μg of penicillin, 80 μg of streptomycin.