Soluble PS effects on AV-C1P transfer by ACD11 and CPTP. Traces in each panel represent AV-SL emission intensity measured at 415 nm as a function of time resulting from loss of AV-SL/Per-PC FRET as AV-SL is transferred to lipid acceptor POPC vesicles. a and b, effect of dihexanoyl-PS (0 to 10 mol %) on AV-C1P transfer by ACD11 and CPTP (2 μg; ∼0.1 nmol), respectively, when the dihexanoyl-PS is included as a lipid-donor vesicle component. c and d, effect of dihexanoyl-PS on AV-C1P transfer by ACD11 and CPTP (2 μg; ∼0.1 nmol), respectively, when the dihexanoyl-PS is included in the buffer (2.5-ml volume) at the indicated concentrations. The critical micelle concentration of dihexanoyl-PS is ∼700 μm.