Table 1. Comparison of parameters of the four methods for the isolation and purification of islet cell clumps (6 experiments/group).
| Parameters | MCT group | Ficoll-400 group | 1077 group | HPU group |
|---|---|---|---|---|
| Duration (±SD, min) | 12 (3) | 34 (3)* | 41 (4)* | 30 (4)* |
| Islet viability | 98.3% | 96.5% | 96.9% | 99.0% |
| Islet purity | 90.4% | 87.8% | 89.1% | 98.6%* |
| Insulin release index (±SD) | 4.5 (0.2) | 3.2 (0.4)* | 3.5 (0.3)* | 4.7 (0.1) |
| Number of islet clumps (±SD, clumps/mouse) | 144 (21) | 149 (32) | 157 (36) | 223 (27)** |
| Recovered islet count per duration (/min) | 12 | 4.4** | 3.8** | 7.4* |
| QIRRPTP | 181 (13) | 212 (36)* | 194 (25) | 238 (31)* |
| Cost/100 islet clumps (RMB/Yuan) | 40 | 70* | 70* | 40 |
| Special reagents/equipment | centrifuge tube, strainer mesh | Ficoll-400 | 1077 separation solution | Sterile stereomicroscope |
Note:
* indicates P < 0.05;
** indicates P < 0.01.
QIRRPTP, the quantity of islets recovered to the required per transplantation procedure.