Table 1. Peptides from βPG identified in Arabidopsis, cannabis and maize.
| Cannabis sativa hypocotyls | ||||||
| gi|156080210 (EST) polygalacturonase non-catalytic protein (Cannabis sativa) | ||||||
| Obs. | Exp. | Cal. | ppm | Ions | peptide sequence | |
| 1548.69 | 1547.69 | 1547.69 | -2.67 | 101 | K.SFNEGTDKFTGYGK.G + ΔPhe | |
| 1583.71 | 1582.70 | 1582.70 | -0.28 | 77 | K.SSNAEQINFNNYGK.S + ΔPhe | |
| 3000.38 | 2999.38 | 2999.39 | -5.18 | 29 | R.QGGSDQFKNYSPGENIPVDSFRRYSR.D + 2 ΔPhe | |
| Arabidopsis thaliana | ||||||
| Different tissues [20] | ||||||
| gi|1762584 polygalacturonase isoenzyme 1 beta subunit homolog [Arabidopsis thaliana] | ||||||
| Dataset | Obs. | Exp. | Cal. | Da | Ions | peptide sequence |
| 3322 | 1205.8 | 2409.59 | 2408.07 | 1.51 | 70 | K.VNFVNYGQSFNPGSETFTGYGK.G + 2 ΔPhe * |
| 3335 | 1205.01 | 2408.01 | 2408.07 | -0.07 | 46 | K.VNFVNYGQSFNPGSETFTGYGK.G + 2 ΔPhe * |
| 3337 | 1205.69 | 2409.36 | 2408.07 | 1.29 | 77 | K.VNFVNYGQSFNPGSETFTGYGK.G + 2 ΔPhe * |
| Soluble proteome of cell suspension cultures [21] | ||||||
| gi|1762584 polygalacturonase isoenzyme 1 beta subunit homolog [Arabidopsis thaliana] | ||||||
| Obs. | Exp. | Cal. | ppm | Ions | peptide sequence | |
| 679.80 | 1357.59 | 1357.58 | 4.46 | 49 | K.ANVGDDSFSSYAK.D + ΔPhe | |
| Zea mays | ||||||
| dataset Maize_endosperm-10-try-150ug-2D29-122109-LTQ3 (22;23) | ||||||
| gi|195613864 polygalacturonase-1 non-catalytic beta subunit precursor [Zea mays] | ||||||
| Fraction | Obs. | Exp. | Cal. | Da | Ions | peptide sequence |
| 20 | 553.59 | 1657.75 | 1657.65 | 0.098 | 40 | R.DDGNVGDDRFTSYAK.G + ΔPhe |
| 829.75 | 1657.49 | 1657.65 | -0.16 | 48 | R.DDGNVGDDRFTSYAK.G + ΔPhe | |
| 23–25 | 1117.84 | 2233.67 | 2233.22 | 0.44 | 108 | R.SFASYSQEANHGENGFSGYGK.N + 2 ΔPhe |
| 745.83 | 2234.47 | 2233.22 | 1.25 | 60 | R.SFASYSQEANHGENGFSGYGK.N + 2 ΔPhe | |
| 27 | 326.11 | 650.21 | 649.31 | 0.89 | 30 | K.SGVDFK.G + ΔPhe |
| 28 | 693.23 | 2076.67 | 2076.19 | 0.48 | 46 | FRSYGAGGNAGVDTFKNYR + 2 ΔPhe |
| dataset Maize juvenileleaf-1-Try-3mg-MCX-1500ugCeO2-Elu-2d19-030210-LTQ3 (22;23) | ||||||
| gi|195613864 polygalacturonase-1 non-catalytic beta subunit precursor [Zea mays] | ||||||
| Fraction | Obs. | Exp. | Cal. | Da | Ions | peptide sequence |
| 14 | 554.13 | 1659.28 | 1657.65 | 1.63 | 28 | R.DDGNVGDDRFTSYAK.G + ΔPhe |
| 830.12 | 1658.23 | 1657.65 | 0.58 | 43 | R.DDGNVGDDRFTSYAK.G + ΔPhe | |
| 17/18 | 1117.86 | 2233.71 | 2233.22 | 0.48 | 80 | R.SFASYSQEANHGENGFSGYGK.N + 2 ΔPhe |
* For these peptides the accepted mass error on the original data surpasses the mass shift induced by the modification, making that partial modification was found. Manual inspection of the MS/MS spectra however indicates that both phenylalanines are modified (Figure A in S1 File), the result presented corresponds to the search with a corrected precursor mass.