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. 2017 Feb 16;12(2):e0172042. doi: 10.1371/journal.pone.0172042

Fig 2. NIH-3T3 cells express FAP-tagged CXCR4 and US27 receptors.

Fig 2

(A) NIH-3T3 mouse fibroblasts were stably transfected with plasmids encoding αFAP-tagged CXCR4 and βFAP-tagged US27. Cells were labeled with membrane impermeable α and β fluorogens, and surface expression of each receptor measured via flow cytometry. The red histogram represents CXCR4, blue histogram is US27, and black histogram is unstained cells. (B) Cells were seeded in a glass-bottom dish, labeled with membrane impermeable α and β fluorogens, and imaged immediately using a Zeiss LSM700 laser scanning confocal microscope. Scale bar, 20 μm. (C) Cells were labeled with Fluo-4 AM calcium indicator dye, then stimulated with either PBS or 100 ng/ml CXCL12/SDF and fluorescence intensity was measured with flow cytometry. RFU = relative fluorescence units. Error bars represent standard error of three replicate experiments.