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. 2017 Feb 16;12(2):e0172042. doi: 10.1371/journal.pone.0172042

Fig 3. US27 increases CXCL12-induced endocytosis of CXCR4.

Fig 3

(A) Confocal images of 3T3-CXCR4 and 3T3-CXCR4/US27 cells grown in glass-bottom dishes and labeled with 100 nM membrane impermeable αRED fluorogen after treatment with 100 ng/ml CXCL12. Scale bar, 10 μm. (B) Total intracellular fluorescence of cells quantified from images taken every 5 minutes (a subset of which is shown in A) with Image J software. Error bars are standard error from average measurement of five cells. * indicates p<0.05, Student t test.