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. 2017 Feb 16;12(2):e0172042. doi: 10.1371/journal.pone.0172042

Fig 4. US27 is rapidly and constitutively internalized independent of CXCR4 or CXCL12.

Fig 4

(A) Live cell imaging after labeling with 100 nM membrane impermeable βRED and treatment with either PBS or 100 ng/ml CXCL12. Scale bar, 20 μm. (B) Quantification of US27 intracellular fluorescence levels in images acquired every five minutes in 3T3-US27 (gray) or 3T3-CXCR4/US27 cells (black). (C) Comparison of US27 (gray) and CXCR4 (black) internalization rates in 3T3-CXCR4/US27 cells. Error bars are standard error from average measurement of five cells.